Volume 8, Issue 1About the featured image
Confocal microscopy of MSCs seeded on fibers scaffolds prepared by Forcespinning®. Cells were stained with DiOC6 (green color) and propidium iodide (red color)—top left, top right, bottom middle panels. Depth color projection was used to visualize distribution of the cells in scaffolds (top middle, bottom left, bottom right panels). Cells penetrated to the depth more than 200 µm in scaffold Sample 1 (50% PCL, G20 orifice) (top left and middle panels) In the case of Sample 2—50% PCL, G30 orifice, classic deposition (top right and bottom left panels); and Sample 3—50% PCL, G30 orifice, CVD (bottom middle and right panels) the penetration was considerably smaller. The micrograph shows in top left, right, and bottom middle panels mesenchymal stem cells stained with DiOC6 for membranes (green) and propidium iodide for nuclei (red). Top middle, bottom left, and right panels represent color-coded depth profile of cells on scaffolds.